Synergistic Effect of Moringa Leaf Extract (Moringa Oleifera Lam) and Purwoceng (Pimpinella alpine Molk) Supplementation on the Quality of Rural Chicken Semen
DOI:
https://doi.org/10.32585/bjas.v4i1.2309Abstract
This study was conducted to examine the synergistic effects of moringa oleifera and purwoceng (Pimpinella alpine molk) supplementation on the quality of chicken semen. In this study used roosters aged about 1 year weighing an average of 2.0 kg as much as 18 tails divided into 6 treatments, each 3 repeats, each repeat consists of 1 tail. The first group of chickens were used as controls, not given treatment, only given basal feed (T0). The second group of chickens were given moringa leaf extract as much as 1 gram / liter of drinking water (T1), chicken group 3 was given purwoceng extract as much as 1 gram / liter of drinking water (T2). Furthermore, chickens in group 4 (T3) were given moringa leaf extract supplements of 250 mg / liter of drinking water and 750 mg / liter per head per day and the 5th group (T4) were given moringa extract supplements as much as 500 mg / liter of drinking water and purwoceng 500 mg / L of drinking water ml every day (T5). The last group (T6) supplemented moringa leaf extract of 750 mg/L of drinking water and purwoceng of 250 mg/l of drinking water. Basal food in this study used BR-1 chicken feed produced by PT. Charoen Pokphand. As a pre-treatment adjustment the chicken is maintained 1 week with basal feed. Furthermore, chickens began to be treated by supplementation of moringa leaves, purwoceng and the mixture. On the 7th day after treatment, semen begins to be taken and tested. Semen collection is done every 3 days with the amount of 3 times the taking. The observed variables were semen volume per ejaculation, ejaculatory spermatozoa count (cells/ml), percentage of living sperm, motility, mass movement, color, viscosity and pH of semen. Results from semen volume and sperm count, motility and percentage of living sperm were analyzed with variance analysis using the spss series 25 program, while viscosity, color and pH were reported in a descriptive manner. From the results of observations it can be concluded that the synergistic effect between moringa leaf extract and purwoceng extract only occurs in the increase in the concentration of spermatozoa, but does not occur in other fractions. Supplementation of moringa leaf extract and its combination with purwoceng extract can increase the production and quality of chicken semen, but the influence of moringa leaf extract is more dominant than purwoceng extract.
Keywords: Rural Chicken, Moringa oleifera, purwoceng (Pimpinella alpine Molk, semen quality
References
DAFTAR PUSTAKA
Abu A. H , Ahemen T and Ikpechukwu P. 2013. The Testicular Morphometry and Sperm Quality of Rubbit Bwoks Fed Graded Levels of Moringa oleifera Leaf Meal (MOLM). Agrosearch (2013) 13 No.1: 49 – 56
Bah, G. S, S.U.R. Chaudhari, J.D. Al-Amin, (2001). Semen characteristics of local breeder cocks in the Sahel region of Nigeria= Caractéristiques du sperme de coqs d’élevage locaux de la région sahélienne du Nigeria= Caracteristicas del semen de gallos de cria locales en la region sahariana en Nigeria. Revue d’élevage et de Médecine Vétérinaire Des Pays Tropicaux, 54(2). : 153-158
Bindari, Y.R., Sulochana S., Nabaraj S., & Tara N.G. (2013). Effects of Nutrition on reproduction-A review. Adv. Appl. Sci. Res. 4(1);421-429.
Darwati, I dan Ika R., 2006. Status Penelitian Purwoceng (Pimpinella alpine Molk) Di Indonesia. Buletin Plasma Nutfah. Vol 12 (1) : 9-15.
Desy Firmalia, I., Yusriani, and Andi Asrina (2021). Pengaruh Edukasi Tentang Pemanfaatan Daun Kelor (Moringa Oleifera) Terhadap Perilaku Ibu Hamil Anemia Di Puskesmas Polongbangkeng Utara Kabupaten Takalar Tahun 2020. Window of Public Health Journal : 844–852.
Garner, D.L. and E.S.E. Hafez. 2000. Spermatozoa and Seminal Plasma. In: Reproduction in Farm Animal. 7th Edited. Lippincott Williams and Wilkins; Maryland. USA
Isnaini, N. 2000. Kualitas Semen Ayam Arab dalam Pengencer NaCl fisiologis dan Ringers pada Suhu Kamar. J. Habitat (11): 233 – 237.
Junaedi, R. I. Afrianti, C. Sumantri dan A. Ginawan. 2016. Penggunaan Dimethyl Sulfoxide sebagai Kriopretektan dalm Pembekuan Semen Ayam Kampung. Jurnal Veteriner, 17 (2): 300-308
Juniarto, A.Z. 2004. Perbedaan pengaruh pemberian ekstrak Eurycoma longifolia dan Pimpinella alpina pada spermatogenesis tikus Spragul Dawley. Tesis. Pascasarjana Ilmu Biomedik Universitas Diponegoro, Semarang. 63 hlm.
Kismiati, S. 1997. Pengaruh Interval Inseminasi Terhadap Peforman Reproduksi dan Heritabilitas Pertumbuhan Ayam Kedu Hitam. Tesis Magister Pertanian. Program Pasca Sarjana Universitas Gajah Mada, Yogyakarta.
Kosin, A.M. 1992. Efek androgenik dan anabolik ekstrak akar Pimpinella alpina Molk. (purwoceng) terhadap anak ayam jantan. Skripsi. FMIPA, Universitas Pakuan Bogor. 61 hlm.
Krisnadi, A. D., 2015. Kelor, Super Nutrisi, E Book, Kelorina. Com. Pusat Informasi dan Pengembangan Tanaman Kelor Indonesia, LSM Media Peduli Lingkungan. Blora, Indonesia
Kusmardika, D. A. (2020). Potensi Aktivitas Antioksidan Daun Kelor (Moringa oleifera) Dalam Mencegahan Kanker. Journal of Health Science and Physiotherapy, 2(1), 46–50.
Lalas, S. and Tsaknis, J. 2002. Extraction and identification of natural antioxidants from the seeds of Moringa oleifera tree variety of Malavi. J. Am. Oil Chem Soc., 79: 677-683
Louis, G.F., Lewis, A.J., Weldon, W.C., Miller, P.S., Kittok, R.J., Stroup, W.W., 1994. The effect of protein intake on Boar libido, semen characteristics, and plasma hormone concentrations. Journal of Animal Science 72(8):2038-2050
Moyo, B. Masika, P. J. Hugo, A. Muchenje, V., 2011. Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. African J. Biotech.Vol 10 (60): 12925-12933
Mustafa, M., Dasrul, D., Yaman, M. A., Wahyuni, S., & Sabri, M. (2017). Pengaruh Pemberian Kombinasi Pakan Fermentasi dengan Multi Enzim dan Vitamin E dalam Ransum terhadap Peningkatan Kualitas Semen Ayam Arab. Jurnal Agripet, 17(1), 43–52.
Nataamijaya, A. G., Setioko, A. R., Brahmantiyo, B., Diwyanto, K., 2003. Performans dan karakteristik tiga galur ayam lokal (pelung, arab, dan sentul). Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner 2003.
Pratiwi, N., Yusuf, T. L., Arifiantini, I., & Sumantri, C. (2019). Kualitas Spermatozoa dalam Modifikasi Pengencer Ringer Laktat Kuning Telur dengan Tambahan Astaxanthin dan Glutathione pada Tiga Jenis Ayam Lokal. Acta Veterinaria Indonesiana, 7(1), 46–54.
Rasyaf M. 1992. Produksi dan Pemberian Pakan Unggas. Hlmn 42-50. Kanisius. Yoyakarta
Sarwono B. 1995. Berternak Ayam Buras.halmn 243-244.. Penebar Swadaya. Jakarta.
Satria, Edy Wahyu (2017) Respon Pemberian Tepung Daun Kelor (Moringa Oliefera) Dalam Pakan Terhadap Penampilan Produksi Dan Kualitas Telur Ayam Petelur. Magister thesis, Universitas Brawijaya.
Sopiyana, S, S. Iskandar, T. Susanti dan D. Yogaswara. 2006. Pengaruh Krioprotektan DMA, DMF dan Glycerol Pada Proses Pembekuan Semen Ayam Kampung . Seminar Nasional Teknologi Peternakan Dan Veteriner.
Suripta. H dan Puji. A., 2021. Peningkatan produksi semen ayam kampung melalui suplementasi daun kelor (Moringa oleifera). Agrisaintifika. Jurnal Ilmu-ilmu Pertanian. Vol 5 (2) : 194-204.
Syarifuddin, N,A, A.L., Toleng , DP. Rahardja , Ismartoyo , M. Yusuf..2016. Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Lambung Mangkurat 180 Daun Kelor Sumber Mineral Seng (Zn) Untuk Meningkatkan Libido Dan Kualitas Semen Pejantan Sapi Bali. Prosiding Seminar Nasional Lahan Basah. Jilid 1: 180-186.
Taufiqqurrachman. 1999. Pengaruh ekstrak Pimpinella alpina Molk. (purwoceng) dan akar Eurycoma longifolia Jack. (pasak bumi) terhadap peningkatan kadar testosteron, LH, dan FSH serta perbedaan peningkatannya pada tikus jantan Spragul Dawley. Tesis. Pascasarjana Ilmu Biomedik, Universitas Diponegoro, Semarang. 119 hlm.
Tolihere, M. R. 1985. Fisiologi Reproduksi pada Ternak. Angkasa, Bandung.
Toelihere, M.R.,1993. Inseminasi Buatan pada Ternak. Angkasa. Bandung
Wijayanti, D. C, N. Isnani dan P. Trisnuwati. 2013. Pengaruh Lama Simpan Semen Dalam NaCl Fisiologis Pada Suhu Kamar Terhadap Kualitas Spermatozoa Ayam Kampung. Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary, 7 (1): 53-55.
Widhyari, S.D A. Esfandiari, A. Wijaya, R. Wulansari, S. Widodo, L. Maylina. 2015. Tinjauan Penambahan Mineral Zn Dalam Pakan Terhadap Kualitas Spermatozoa Pada Sapi Frisian Holstein Jantan. Jurnal Ilmu Pertanian Indonesia (JIPI). Vol. 20 (1): 72 77.
Young, S. S., B. Eskenazi , F. M. Marchetti G. Block A. J Wyrobek. 2008. The association of folate, zinc and antioxidant intake with sperm aneuploidy in healthy non-smoking men. Human Reproduction, Volume 23(5):1014–1022,
Zambrano E., GL Rodriguez‐Gonzalez, C Guzman, R Garcia‐Becerra, L Boeck, L Diaz, M Menjivar, F Larrea, PW Nathanielsz. 2005. A Maternal Low Protein Diet During Pregnancy and Lactation in the Rat Im-Pairs Male Reproductive Development. Journal of Physi-ology, 563, 275-284.
