The Effect of Young Timor Deer (Rusa timorensis) Hornbill Flour Supplementation on the Testicular Size of Male Wistar Rats (Rattus norvegicus)
DOI:
https://doi.org/10.32585/bjas.v7i2.7226Keywords:
Timor deer, Young deer antlers, Mice, Supplementation, Reproductive organsAbstract
The processing of young deer antlers exemplifies the use of deer as wildlife, guided by the principle of continuity and without harming them. The purpose of this study was to determine the effect of young Timor deer antler meal and to determine the impact of its supplementation on the size of the testes of male Wistar rats. Twenty male Wistar rats with a body weight of 200–250 g and an age of 90 days were used. The samples were divided into four groups: a control group and three treatment groups of young Timor deer antler meal at doses of 25 mg kg-1 BW-1, 50 mg kg-1 BW-1, and 100 mg kg-1 BW-1. Ca and Zn measurements were carried out using an atomic absorption spectrophotometer at the Feed Nutrition Laboratory of Diponegoro University. The size of the left and right testes (length, width, diameter, and weight) was analyzed using Analysis of Variance, and Duncan's Multiple-Range Test was used for testis weight. Data on Ca and Zn content were analyzed descriptively. Therefore, it can be concluded that supplementation with young Timor deer antler flour affected testicular weight, but not testicular length, width, and diameter. The results showed a significant difference (P<0.05) in testicular weight parameters, and testicular length, width, and diameter were not significantly different. Therefore, it can be concluded that supplementation with young Timor deer antler flour affected testicular weight, but not testicular length, width, and diameter.
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Amin, M., Aryadin Putra, R., Suhardiani, A., Andriati, R., Yanuarianto, O., Hidayat Dilaga, S., Nur Muhamad, dan, Studi Teknologi Pakan Ternak Jurusan Peternakan, P., Negeri Jember Jl Mastrip Jember, P., Timur, J., kunci, K., Pakan, H., & Bali, S. (n.d.). Kandungan Mineral Hijauan Pakan Yang Diberikan kepada Sapi Bali di Pulau Lombok (Mineral Content of Forage Feed Given to Bali Cattle in Lombok Island). Jurnal Ilmu Dan Teknologi Peternakan Indonesia, 9(1), 1–8.
Atang and E S. Palupi. 2020. Pengaruh antioksidan alami berat testis mencit (Mus musculus) yang terpapar senyawa kimia plastic. J. Research of Empowerment and Development. 1: 43-48.
Berger, T. (2019). Testicular estradiol and the pattern of Sertoli cell proliferation in prepuberal bulls. Theriogenology, 136, 60–65. https://doi.org/10.1016/j.theriogenology.2019.06.031
Chen, J., Yang, Y., Abbasi, S., Hajinezhad, D., Kontulainen, S., & Honaramooz, A. (2015). The Effects of Elk Velvet Antler Dietary Supplementation on Physical Growth and Bone Development in Growing Rats. Evidence-Based Complementary and Alternative Medicine, 2015. https://doi.org/10.1155/2015/819520.
Dé, F., Chalmel, R., Rolland, A. D., Niederhauser-Wiederkehr, C., Chung, S. S. W., Demougin, P., Gattiker, A., Moore, J., Patard, J.-J., Wolgemuth, D. J., Jé Gou †, B., & Primig, M. (2007). The conserved transcriptome in human and rodent male gametogenesis. www.pnas.org/cgi/content/full/
Fitria, L., Mulyati., C.M. Tiraya and A.S. Budi. 2015. Profil reproduksi tikus (Rattus norvegicus) Berkenhout, galur Wistar stadia muda, pradewasa dan dewasa. J. Biologi Papua. 7 (1): 29-36.
Ismanto, A. 2016. Hidrolisis protein tanduk muda rusa sambar (Rusa unicolor) serta potensinya sebagai penurun hipertensi. J. Tropical Pharmaeutical and Chemistry. 3 185 – 190.
Gao, D., et al. (2021). Deer antler velvet: Ethnopharmacology and therapeutic potential. Journal of Ethnopharmacology, 267, 113549.
Dé, F., Chalmel, R., Rolland, A. D., Niederhauser-Wiederkehr, C., Chung, S. S. W., Demougin, P., Gattiker, A., Moore, J., Patard, J.-J., Wolgemuth, D. J., Jé Gou †, B., & Primig, M. (2007). The conserved transcriptome in human and rodent male gametogenesis. www.pnas.org/cgi/content/full/
Guan, Y., & Martin, G. B. (2017). Cellular and molecular responses of adult testis to changes in nutrition: Novel insights from the sheep model. In Reproduction (Vol. 154, Issue 5, pp. R133–R141). BioScientifica Ltd. https://doi.org/10.1530/REP-17-0061
Junqueira, L.C., J. Carneiro and R.O. Kelley. 2007. Histologi Dasar. Ed. 5th. EGC: Jakarta.
Lea, M C, S C Becker-Silva, H Chiarini-Garcia, L R Franca. 2014. Sertoli cell efficiency amd daily sperm production in goats (Capra hircus). Animal Reproduction. 1 122-1128.
Liu, G., Liu, D., Zhu, M., Zhang, M., Li, C., Xu, X., & Pan, F. (2025). Insulin-like growth factor-1 promotes the testicular sperm production by improving germ cell survival and proliferation in high-fat diet-treated male mice. Andrology, 13(2), 342–358. https://doi.org/10.1111/andr.13645
Mahre, M B, H Wahid, Y Rosnina, F F A Jesse, A Z Jaji, N A Ojo. 2016. Anatomy of the female repro-ductive system of Rusa deer (Rusa timorensis). So-koto Journal of Veterinary Sciences. 14 15-20.
Mirnamniha, F Faroughi, E Tahmasbpoura, P Ebrahimi and A B Harchegani, 2019. An overview on role of some trace elements in human reproductive health, sperm function and fertilization process. Reviews on Environmental Health, 0 1-10.
Mirsky, M. L., S. Portugal., H. Pisharath., J. L. Osowski & L. Kearney. Utility of Orchidometric parameters for assesing sexual maturation in male Cynomolgus macaques (Macaca fascicularis). Comparative Medicine. 66(6), 480-488.
Orassay, A., Sadvokassova, D., Berdigaliyev, A., Sagintayev, A., Myrzagali, S., Omarova, Z., Toktarov, N., Liu, D., & Xie, Y. (2024). Deer antler extract: Pharmacology, rehabilitation and sports medicine applications. In Pharmacological Research - Modern Chinese Medicine (Vol. 10). Elsevier B.V. https://doi.org/10.1016/j.prmcm.2023.100316
Pratiwi, A., Suryanto, B., & Arifin, Z. (2020). Morphometric analysis of reproductive organs in local goats using vernier caliper. Jurnal Ilmu Ternak, 20(2), 89–96.
Qamar, A. Y., Naveed, M. I., Raza, S., Fang, X., Roy, P. K., Bang, S., Tanga, B. M., Saadeldin, I. M., Lee, S., & Cho, J. (2023). Role of antioxidants in fertility preservation of sperm - A narrative review. Animal Bioscience, 36(3), 385–403. https://doi.org/10.5713/ab.22.0325
Sudibyo, M, Y Santosa, B Masy’ud and T Toharmat. 2013. Preferences of Rusa timorensis to grasses and their body morphometry and Ranggah Muda Rusa antler characteristics. Media Peternakan. 143-151.
Sudrajad, P and B. Utomo. 2021. The effect of suplemntary feeding on the reproductive performance of beef cows in supporting the siwab program. Bantara Journal of Animal Science. 3 (1): 37-44.
Supriyadi, A., Widyastuti, E. S., & Suryani, E. 2018. Pengaruh suplementasi mineral organik terhadap kecernaan bahan kering dan bahan organik pakan pada sapi potong. Jurnal Ilmu Ternak, 18(2), 75–82.
Susetyarini, RR. E. 2015. Jumlah sel spermiogenesis tikus putih yang diberi tannin daun beluntas (Pluchea indica) sebagai sumber belajar. Seminar Nasional XII Pendidikan Biologi FKIP UNS.
Syamyono, O., D. Samsudewa dan E.T. Setiatin. 2014. Korelasi lingkar skrotum dengan bobot badan, volume semen, kualitas semen dan kadar testosterone pada kambing Kejobong muda dan dewasa. Buletin Peternakan. 38 (3): 132-140.
Takadjandji, M, A Rianti and C Sudaryo. 2019. The economic value of Ranggah Muda Rusa from Timor deer captive breeding at Dramaga Forest Research. Proceedings of the IOP Conference Series: Earth and Environmental Science. 1-12.
Tansathien, K., Suriyaaumporn, P., Charoenputtakhun, P., Ngawhirunpat, T., Opanasopit, P., & Rangsimawong, W. (1207). Development of Sponge Microspicule Cream as a Transdermal Delivery System for Protein and Growth Factors from Deer Antler Velvet Extract. In Biol. Pharm. Bull (Vol. 42, Issue 7).
Toelihere, M.R. 1993. Fisiologi Reproduksi pada Hewan Ternak. Angkasa, Bandung.
Wu F, H Li, L Jin, X Li, Y Ma, J You, S Li, Y Xu. 2013. Deer antler base as a traditional Chinese medicine : A review of its traditional uses, chemistry and pharmacology. J. Ethnopharmacology. 145 403 – 415.
Xu, Y., Han, C. Y., Park, M. J., & Gye, M. C. (2022). Increased testicular insulin-like growth factor 1 is associated with gonadal activation by recombinant growth hormone in immature rats. Reproductive Biology and Endocrinology, 20(1). https://doi.org/10.1186/s12958-022-00944-z.
Zang, Z. J., Tang, H. F., Tuo, Y., Xing, W. J., Ji, S. Y., Gao, Y., & Deng, C. H. (2016). Effects of velvet antler polypeptide on sexual behavior and testosterone synthesis in aging male mice. Asian Journal of Andrology, 18(4), 613–619. https://doi.org/10.4103/1008-682X.166435
Zhang, B X, C A Jin dan Y P Zhao. 2005. The chemical composition and utilization of antler plate. Special Economic Animal and Plant 12 7.
Zang, Z. J., Tang, H. F., Tuo, Y., Xing, W. J., Ji, S. Y., Gao, Y., & Deng, C. H. (2016). Effects of velvet antler polypeptide on sexual behavior and testosterone synthesis in aging male mice. Asian Journal of Andrology, 18(4), 613–619. https://doi.org/10.4103/1008-682X.166435
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