Enzymes as Potencial Source for Clean Label Bakery Product: Part 1, Mechanism and Application Single Enzym
DOI:
https://doi.org/10.32585/jfap.v2i2.2708Abstract
Background: Increased public awareness of consuming healthy food has driven bakery industry to applied production methods and components of food products that are tailored to the market needs. Food product can be positioned as natural, organic, or free from additives/ preservatives which often referred to clean label trend. Bakery industry commonly using chemical emulsifier as component which improve characteristic and quality baked goods. Usage of chemical component is not appropriate with perception of clean label, although it is not yet clear what a clean label exactly means. Chemical emulsifier has potentially negative effect to health such as intestinal inflammation, obesity, metabolic syndrome and glucose resistance based on several research. Food enzyme can be alternative to replace chemical emulsifier and potentially source of clean label bakery product. Therefore, sustainable study was needed to find role single enzym as food additive and processing aid in bakery product application.
Scope and approach: This review explain about the role single enzyme application in bakery product which discuss under three main headings include (i) enzyme as food additive and processing aid, ii) Characteristic enzyme to improve bakery product processing (dough mixing, fermentation, baking), sensories properties and appearance iii) Enzyme mechanism and application to enhance bakery product quality. Optimization of the role and function of enzymes can be conduct by enzyme quality validation through baking tests including formulation development, process parameters (dough rheology, handling machine and baking parameter), product appearance and sensory characteristics.
Key findings and conclusion: Food enzymes play a role in enzymatic modifications as biodegradable proteins which not affected to nutritional value baked goods. Enzyme technology is a clean process with low energy consumption, low waste production, safe and less toxic working environment. Therefore, enzyme has potential to fulfill clean label trends and encourage researchers and developers in food industry to explore potential use of food enzymes in bakery products. Enzymes which usually used in bakery come from hydrolase class (amylase, protease, hemicellulase, lipase, xylanase and asparaginase), oxidoreductase class (lipoxygenase and glucose oxidase) and transferase class (transglutaminase). Application enzymes in bakery processs have their respective roles according to enzymes specific characteristics. Enzymes has the main role such as improve rheological and functional properties of dough according to baked goods type, enhance quality and characteristics baked goods including volume, crumb texture, color, taste and extend shelf life (antistaling). Sustainable research and development was needed to optimize the role of enzyme in baked goods by several approach such as (i) incorporation enzymes with other ingredients in the food matrix, (ii) parameters which affect to the work of enzymes in food systems (iii) potential of enzyme combinations to improve baked goods quality and (iv) understanding of usage regulation enzymes as food additives and food processing.
Keyword: enzyme, clean label, food additive, processing aid, bakery product
References
Amigo, J.M., del Olmo, A., Engelsen, M.M, Lundkvist, H., dan Engelsen, S.B. 2021. Staling of white wheat bread crumb and effect of maltogenic α-amylases. Part 3: Spatial evolution of bread staling with time by near infrared hyperspectral imaging. Food Chemistry, 353: 129478.
Amiri, A., Shahedi, M., dan Kadivar, M. 2016. Evaluation of physicochemical properties of gluten modified by Glucose oxidase and Xylanase. Journal of Cereal Science, 71: 37–42.
Anese, M., Quarta, B. dan Frias, J. 2011. Modelling the effect of asparaginase in reducing acrylamide formation in biscuits. Food Chemistry, 126: 435-440.
Aponso, M.M.W, De Silva, G.O dan Abeysundara, A.T. 2017. Emulsifiers as food additives: An overview on the impact to obesity and gut diseases. Journal of Pharmacognosy and Phytochemistry, 6(3): 485-487.
Asioli, D., Aschemann-Witzela, J., Caputo, V., Vecchio, R., Annunziata, A., Naes, T., dan Varela, P. 2017. Making sense of the “clean label” trends: A review of consumer food choice behavior and discussion of industry implications. Food Research International.
Aschemann-Witzel, J., Varela, P., dan Peschel, A.O. 2019. Consumers categorization of food ingredients: Do consumers perceive them as ‘clean label’ producers expect? An exploration with projective mapping. Food Quality and Preference, 71: 117-128.
Bahal, G., Sudha, M. L. dan Ramasarma, P. R. 2013. Wheat germ lipoxygenase: Its effect on dough rheology, microstructure, and bread making quality. International Journal of Food Properties, 16(8): 1730–1739.
Bakery market by product (bread, rolls, cakes, pastries, biscuits, pies, and others) by type (snacks, breakfast, hybrids, and specialty), by distribution channel (hypermarkets, supermarkets, specialty stores, and online) global opportunity analysis and industry forecast, 2020–2030. https://www.nextmsc.com. https://www.nextmsc.com/report/bakery-market. Diakses pada 13 Juli 2022.
Baking enzymes market size, share and industry analysis report by product (proteases, lipases, carbohydrases [amylases, xylanases, cellulases, pectinases, lactases], polymerases & nucleases, phytases, catalyses) and application (bread, cake, biscuit), regional outlook, growth potential, competitive market share & forecast, 2021–2027. https://www.gminsights.com. https://www.gminsights.com/industry-analysis/baking-enzymes-market. Diakses pada 13 Juli 2022.
Bauer, N., Koehler, P., Wieser, H., dan Schieberle, P. 2003. Studies on effects of microbial transglutaminase on gluten proteins of wheat. I. Biochemical analysis. Cereal Chem, 80(6): 781–786.
Bonet, A., Rosell, C.M., Caballero, P.A., Gomez, M., Pe´rez-Munuera, I., dan Lluch, M.A. 2006. Glucose oxidase effect on dough rheology and bread quality: A study from macroscopic to molecular level. Food Chemistry, 99: 408–415.
Buche, F., Davidou, S., Pommet, M., Potus, J., Rouillé, J., Verté, F., dan Nicolas, J. 2011. Competition for Oxygen Among Oxidative Systems During Bread Dough Mixing: Consequences of Addition of Glucose Oxidase and Lipoxygenase on Yeasted Dough Rheology. Cereal Chem., 88 (5) :518–524.
Butt, M.S., Tahir-Nadeem, M, Ahmad, Z. dan Sultan, M.T. 2008. Xylanases and their applications in baking industry. Food Technol. Biotechnol, 46 (1): 22–31.
Caballero, P.A., Gomez, M., dan Rosell, C.M. 2007. Improvement of dough rheology, bread quality and bread shelf-life by enzymes combination. Journal of Food Engineering, 81: 42–53.
Capuano, E., Ferrigno, A., Acampa, I., Serpen, A., Açar, O.C., Gökmen, V., dan Fogliano, V. 2009. Effect of flour type on Maillard reaction and acrylamide formation during toasting of bread crisp model systems and mitigation strategies. Food Research International, 42: 1295–1302.
Chandrasekaran, M. 2016. Enzymes in Food and Beverage Processing. CRC Press, USA.
Chassaing, B, Van de Wiele, T., De Bodt, J., Marzorati, M. dan Gewirtz, A.T. 2017. Dietary emulsifiers directly alter human microbiota composition and gene expression ex vivo potentiating intestinal inflammation. Gut Published Online First, 1-14.
Colakoglu, A.S. dan Ozkaya, H. 2012. Potential use of exogenous lipases for DATEM replacement to modify the rheological and thermal properties of wheat flour dough. Journal of Cereal Science, 55: 397-404.
Collins, T., Hoyoux, A., Dutron, A., Georis, J., Genot, B., Dauvrin, T., Arnaut, F., Gerday, C., dan Feller, G. 2006. Use of glycoside hydrolase family 8 xylanases in baking. Journal of Cereal Science, 43: 79–84.
Collar, C., & Bollain, C. 2005. Impact of microbial transglutaminase on the staling behaviour of enzyme supplemented pan breads. European Food Research and Technology, 221(3–4): 298–304.
Cox, S., Sandall, A., Smith, L., Rossi, M. dan Whelan, K. 2020. Food additive emulsifiers: A review of their role in foods, legislation and classifications, presence in food supply, dietary exposure, and safety assessment. Nutrition Reviews, 79(6): 726–741.
Csaki, K.F. dan Sebestyen, E. 2019. Who will carry out the tests that would be necessary for proper safety evaluation of food emulsifiers?. Food Science and Human Wellness, 8: 126–135
Dahiya, S., Bajaj, B.K., Kumar, A., Tiwari, S.K., dan Singh, B. 2020. A review on biotechnological potential of multifarious enzymes in bread making. Process Biochemistry, 99: 290–306.
David, I. dan Misca, C. 2012. The monitoring of enzyme activity of protease on the bread dough. Journal of Agroalimentary Processes and Technologies, 18 (3): 236-241.
Deng, L., Wang, Z., Yang, S., Song, J., Que, F., Zhang, H. dan Feng, F. 2016. Improvement of functional properties of wheat gluten using acid protease from Aspergillus usamii. PLoS One, 11.
Garvey, E.C., O'Sullivan, M.G., Kerry, J.P., Milnerd, L., Gallagher, E., dan Kilcawleya, K.N. 2020. Characterising the sensory quality and volatile aroma profile of clean-label sucrose reduced sponge cakes. Food Chemistry.
Goesaert, H., Slade, L., Levine, H., dan Delcour, J.A. 2009. Amylases and bread firming – an integrated view. Journal of Cereal Science, 50: 345–352.
Haghighat-Kharazi, S., Kasaai, M.R., Milani, J.M., dan Khajeh, K. 2020. Antistaling properties of encapsulated maltogenic amylase in gluten-free bread. Food Sci Nutr, 8:5888–5897.
Halmos, E.P., Mack, A., dan Gibson, P.R. 2019. Review article: Emulsifiers in the food supply and implications for gastrointestinal disease. Aliment Pharmacol Ther, 49: 41–50.
Harada, O., Lysenko, E. D. dan Preston, K. R. 2000. Effects of commercial hydrolytic enzyme additives on Canadian short process bread properties and processing characteristics. Cereal Chemistry, 77(1): 70–76.
Heredia-Sandoval, N.G., Valencia-Tapia, M.Y., de la Barca, A.M.C., dan Islas-Rubio, A.R. 2016. Microbial proteases in baked goods: modification of gluten and effects on immunogenicity and product quality. Foods, 5:59
Huang, Z., Brennana, C.S., Zheng, H., Mohana, M.S., Stipkovitsa, L., Liua, W., Kulasiria, D., Guane, W., Zhaoe, H., dan Liue, J. 2020. The effects of fungal lipase-treated milk lipids on bread making. LWT- Food Science and Technology, 128: 109455.
Hsu, P. Y., and Benfey, P. N. 2018. Small but mighty: functional peptides encoded by small ORFs in plants. Proteomics, 18: e1700038.
Jia, C., Huang, W., Abdel-Samie, M.A., Huang, G., dan Huang, G. 2011. Dough rheological, Mixolab mixing, and nutritional characteristics of almond cookies with and without xylanase. Journal of Food Engineering, 105: 227–232.
Joye, I. J., Lagrain, B. dan Delcour, J. A. 2009. Endogenous redox agents and enzymes that affect protein network formation during breadmaking: A review. Journal of Cereal Science, 50(1): 1–10.
Kheng, P.P. dan Omar, I.C., 2005. Xylanase production by a local fungal isolate, Aspergillus niger USM AI 1 via solid state fermentation using Palm Kernel Cake (PKC) as substrate. Songklanakarin J. Sci. Technol, 27: 325–336.
Kieliszek, M. dan Misiewicz, A. 2014. Microbial transglutaminase and its application in the food industry. A review. Folia Microbiology, 59: 241–250.
Kocabas, D.S. dan Grumet, R. 2019. Evolving regulatory policies regarding food enzymes produced by recombinant microorganisms. GM Crops & Food, 10:191–207.
Koga, S., Rieder, A., Ballance, S., Uhlen, A. K., & Veiseth-Kent, E. 2019. Gluten-degrading proteases in wheat infected by Fusarium graminearum—protease identification and effects on gluten and dough properties. Journal of Agricultural and Food Chemistry, 67(40): 11025–11034.
Kashiwagi, T., Yokoyama, K., Ishikawa, K., Ono, K., Ejima, D., Matsui, H. dan Suzuki, E. 2002. Crystal structure of microbial transglutaminase from Streptoverticillium mobaraense. J Biol Chem, 277: 44252– 44260.
Kukurova, K., Morales, F.J., Bedna´rikova, A., dan Ciesarova, Z. 2009. Effect of L-asparaginase on acrylamide mitigation in a fried-dough pastry model. Mol. Nutr. Food Res, 53: 1532–1539.
Laster, J., Bonnes, S.L., dan Rocha, J. 2019. Increased use of emulsifiers in processed foods and the links to obesity. Current Gastroenterology Reports, 21: 61.
Lebesi, D. dan Tzia, C. 2012. Use of endoxylanase treated cereal brans for development of dietary fiber enriched cakes. Innovative Food Science and Emerging Technologies, 13: 207–214.
Li, J., Hou, G.G., Chen, Z., dan Gehring, K. 2013. Effects of endoxylanases, vital wheat gluten, and gum arabic on the rheological properties, water mobility, and baking quality of whole wheat saltine cracker dough. Journal of Cereal Science, 58: 437-445.
Monie, A., David, A., Clemens, K., Malet-Martino, M., Balayssac, S., Perez, E., Franceschi, S., Crepin, M., dan Delample, M. 2020. Enzymatic hydrolysis of rapeseed oil with a non-GMO lipase: A strategy to substitute mono- and diglycerides of fatty acids and improve the softness of sponge cakes. LWT - Food Science and Technology.
Nascimento, K., Paes, S.D., dan Augusta, I.M. 2018. A Review 'Clean labeling': Applications of Natural Ingredients in Bakery Products. Journal of Food and Nutrition Research, 6 (5): 285-294.
Park, S.H., Na, Y., Kim, J., Kang, S.D., dan Park, K. 2017. Properties and applications of starch modifying enzymes for use in the baking industry. Food Sci Biotechnol.
Partridge, D., Lloyd, K.A., Rhodes, J. M., Walker, A. W., Johnstone, A. M. dan Campbell, B. J. 2019. Food additives: Assessing the impact of exposure to permitted emulsifiers on bowel and metabolic health – introducing the FADiets study. Nutrition Bulletin, 44: 329-349.
Popper, L. dan Losche, K. 2021. Understanding Baking Enzymes. Robert Wenzel. Hamburg, Germany
Pourmohammadi, K. dan Abedi, E. 2021. Enzymatic modifications of gluten protein: Oxidative enzymes. Food Chemistry, 356: 129679.
Pourmohammadi, K. dan Abedi, E. 2021. Enzymatic modifications of gluten protein: Oxidative enzymes. Food Chemistry, 356: 129679.
Rahman, M.M. dan Simsek, S. 2020. Go clean label: replacement of commercial dough strengtheners with hard red spring wheat flour in bread formulations. J Food Sci Technol.
Rahman, M.M., Ohm, J., dan Simsek, S. 2022. Clean-label breadmaking: Size exclusion HPLC analysis of proteins in dough supplemented with additives vs hard red spring wheat flour. Journal of Cereal Science, 104: 103426.
Rasiah, I.A., Sutton, K.H., Low, F.L., Lin, H.M. dan Gerrard, J.A. 2005. Crosslinking of wheat dough proteins by glucose oxidase and the resulting effects on bread and croissants. Food Chemistry, 89: 325–332.
Raveendran, S., Parameswaran, B., Ummalyma, S.B., Abraham, A.A., Mathew, A.K., Madhavan, A., Rebello, S., dan Pandey, A. 2018. Food Technol. Biotechnol, 56 (1): 16-30.
Redan, B.W. 2020. Processing aids in food and beverage manufacturing: Potential source of elemental and trace metal contaminants. Journal of Agricultural and Food Chemistry, A-G.
Rizzello, C.G., De Angelis, M., Di Cagno, R., Camarca, A., Silano, M., Losito, I., De Vincenzi, M., De Bari, M., Palmisano, F., Maurano, F., Gianfrani, C. dan Gobbetti, M. 2007. Highly efficient gluten degradation by Lactobacilli and fungal proteases during food processing: new perspectives for celiac disease. Applied and Environmental Microbiology, 73(14): 4499–4507.
Rizzello, C.G., Curiel, J.A., Nionelli, L., Vincentini, O., Di Cagno, R., Silano, M., Gobbetti, M., dan Coda, R. 2014. Use of fungal proteases and selected sourdough lactic acid bacteria for making wheat bread with an intermediate content of gluten. Food Microbiology, 37: 59-68.
Rodríguez-García, J., Sahi, S.S. dan Hernando, I. 2014. Functionality of lipase and emulsifiers in low-fat cakes with inulin. LWT - Food Science and Technology, 58: 173-182.
Rosell, C. M. dan C. Collar. 2008. Effect of various enzymes on dough rheology and bread quality. In Recent Research Developments in Food Biotechnology. Enzymes as Additives or Processing Aids, 165–183. Kerala, India: Research Signpost.
Salgado, C.A., dos Santos, C.I.A. dan Vanetti, M.C.D. 2022. Microbial lipases: Propitious biocatalysts for the food industry. Food Bioscience, 45: 101509.
Schaffarczyk, M., Østdal, H., Koehler, P., 2014. Lipases in wheat breadmaking: Analysis and functional effects of lipid reaction products. J. Agric. Food Chem, 62: 8229-8237.
Simsek, S. 2020. Clean-label bread: Using hard red spring wheat to replace dough improvers in whole wheat bread. J Food Process Preserv, 00: e14920.
Singhania, R.R., Sukumarana, R.K., Patel, A.K., Larroche, C., dan Pandey, A. 2010. Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases. Enzyme and Microbial Technology, 46: 541–549.
Wang, P., Zou, M., Tian, M., Gu, Z., dan Yang, R. 2018. The impact of heating on the unfolding and polymerization process of frozen-stored gluten. Food Hydrocolloids, 85: 195–203.
Whitehurst, R.J. dan van Oort, M. 2010. Enzymes in Food Technology, Second Edition. Blackwell Publishing Ltd, USA.
Wouters, G.B., Rombouts, I., Fierens, E., Brijs, K. dan Delcour, J.A. 2016. Relevance of the functional properties of enzymatic plant protein hydrolysates in food systems. Comprehensive Reviews in Food Science and Food Safety, 15: 786-800.
Yegin, S., Altinel, B., dan Tuluk, K. 2018. A novel extremophilic xylanase produced on wheat bran from Aureobasidium pullulans NRRL Y-2311-1: Effects on dough rheology and bread quality. Food Hydrocolloids, 81: 389-397.
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